The involvement of kisspeptin in centrally regulatory mechanism of neuropeptide Y on testosterone secretion in male Wistar rats

نویسندگان

  • Abdolkarim Hosseini Department of Physiology, Faculty of Biological Sciences, Shahid Beheshti University, Tehran, Iran
  • Homayoun Khazali Department of Physiology, Faculty of Biological Sciences, Shahid Beheshti University, Tehran, Iran
  • Shahrbanoo Oryan Department of Animal Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran
  • Vahid Azizi Department of Animal Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran
چکیده مقاله:

Introduction: Numerous studies have demonstrated that kisspeptin, a peptide from the KISS1 gene, plays an important role in regulating the secretion of gonadotropin releasing hormone (GnRH). Also, there is some evidence suggesting that kisspeptin can interact with other neuropeptides for the control of the reproductive axis. In the present study, we have investigated the effect of central administration of either kisspeptin or neuropeptide Y (NPY) or both on the mean plasma testosterone concentration in male rats. Methods: In this experimental study, 66 male Wistar rats were allocated into 11 groups (n=6 per group) receiving saline, kisspeptin (1 nmol), P234 (kisspeptin receptor antagonist, 1 nmol), NPY (2.3 nmol), BIBP3226 (NPY receptor antagonist, 7.8 nmol) or co-administration of them via intracerebroventricular (ICV) injection at 9:00-9:30 A.M. Blood samples were collected at 30 and 60 min following the injections for hormone assay. The serum testosterone concentration was measured using rat testosterone kit and the method of radioimmunoassay. Results: Kisspeptin or NPY injection significantly increased the mean serum testosterone concentration compared to saline at 30 and 60 min postinjection (P<0.001). The co-injection of kisspeptin+NPY considerably raised the mean serum testosterone concentration compared to NPY in both 30 and 60 min after the administration (P<0.001). This study indicates that P234 or BIBP3226 significantly attenuated (P<0.001) the testosterone increase after the kisspeptin injection compared to kisspeptin while a stimulatory increase effect was observed in the kisspeptin groups compared to either NPY or kisspeptin. Conclusion: Based upon the results, NPY may modulate the testosterone secretion indirectly via the kisspeptin signaling system.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

the involvement of kisspeptin in centrally regulatory mechanism of neuropeptide y on testosterone secretion in male wistar rats

introduction: numerous studies have demonstrated that kisspeptin, a peptide from the kiss1 gene, plays an important role in regulating the secretion of gonadotropin releasing hormone (gnrh). also, there is some evidence suggesting that kisspeptin can interact with other neuropeptides for the control of the reproductive axis. in the present study, we have investigated the effect of central admin...

متن کامل

the effect of aqueous extract of garlic on formalin-induced pain in male rats

چکیده اثر عصاره آبی سیر بر درد ناشی از آزمون فرمالین در موش صحرایی نر به کوشش نرگس اسکندری روزبهانی زمینه و هدف: گیاه سیر از خانواده لیلیاسه و گونهallium sativum بومی آسیای میانه بوده و از دوران ،باستان تاکنون به خواص درمانی متفاوت آن مثل: کاهندگی قندخون، کلسترول خون، فشار خون، اثرات مفیدآن بر دستگاه قلبی عروقی و بیماریهای انعقادی خون، اثرات آنتی اکسیداتیو، درمان بیماریهای تنفسی وگوارشی، ا...

15 صفحه اول

The effect of intracerebroventricular administration of neuropeptide Y on reproductive axis function in the male Wistar rats: Involvement of hypothalamic KiSS1/GPR54 system

Several studies have shown that neuropeptide Y (NPY) is considered to be one of the key regulators of the hypothalamic-pituitary-gonadal axis in the mammals. Also, kisspeptin is a powerful upstream regulator of gonadotropin-releasing hormone neurons in the hypothalamus. The present study aims to investigate the effects of the intracerebroventricular (ICV) injection of NPY and BIBP3226 (NPY rece...

متن کامل

the impact of e-readiness on ec success in public sector in iran the impact of e-readiness on ec success in public sector in iran

acknowledge the importance of e-commerce to their countries and to survival of their businesses and in creating and encouraging an atmosphere for the wide adoption and success of e-commerce in the long term. the investment for implementing e-commerce in the public sector is one of the areas which is focused in government‘s action plan for cross-disciplinary it development and e-readiness in go...

the washback effect of discretepoint vs. integrative tests on the retention of content in knowledge tests

در این پایان نامه تاثیر دو نوع تست جزیی نگر و کلی نگر بر به یادسپاری محتوا ارزیابی شده که نتایج نشان دهندهکارایی تستهای کلی نگر بیشتر از سایر آزمونها است

15 صفحه اول

the effect of taftan pozzolan on the compressive strength of concrete in the environmental conditions of oman sea (chabahar port)

cement is an essential ingredient in the concrete buildings. for production of cement considerable amount of fossil fuel and electrical energy is consumed. on the other hand for generating one tone of portland cement, nearly one ton of carbon dioxide is released. it shows that 7 percent of the total released carbon dioxide in the world relates to the cement industry. considering ecological issu...

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ذخیره در منابع من قبلا به منابع من ذحیره شده

{@ msg_add @}


عنوان ژورنال

دوره 20  شماره None

صفحات  267- 276

تاریخ انتشار 2016-11

با دنبال کردن یک ژورنال هنگامی که شماره جدید این ژورنال منتشر می شود به شما از طریق ایمیل اطلاع داده می شود.

کلمات کلیدی

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023